| Expression pattern: |
DN |
| Associated gene: |
MKK6, MAPK14, FOXC1, U2AF2 |
| Associated microRNA: |
- |
| Biological function: |
Suppresses proliferation, migration/invasion and metastasis; promotes apoptosis in colorectal cancer. |
| Molecular mechanism: |
circMAPK14 encodes a 175-aa peptide (circMAPK14-175aa) that competitively binds MKK6, represses MAPK14 phosphorylation and nuclear translocation, thereby promoting ubiquitin-proteasome-mediated degradation of FOXC1; FOXC1 represses U2AF2 transcription to reduce circMAPK14 biogenesis, forming a positive feedback loop. |
| Biological pathway or process: |
MAPK (inhibits); proliferation (inhibits); apoptosis (promotes); migration (inhibits); invasion (inhibits); metastasis (inhibits); ubiquitination (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; ChIP / ChIP-seq; In Vivo Animal Model; H&E Staining; IF (Immunofluorescence); Bioinformatics Analysis |
| Clinical significance: |
Lower circMAPK14 abundance was associated with tumour size, TNM stage and lymphatic metastasis, and patients with lower circMAPK14 had worse overall survival. |
| Description: |
circMAPK14 (hsa_circ_0131663) is downregulated in colorectal cancer and mainly localized in the cytoplasm. It suppresses CRC proliferation and metastasis by encoding the peptide circMAPK14-175aa, which competes with MAPK14 for MKK6 binding, inhibits MAPK14 nuclear translocation, and promotes FOXC1 ubiquitination/degradation within a FOXC1-U2AF2 positive feedback loop. |
| Confidence score: |
0.8192 |