circRNA basic information
circBase ID: hsa_circ_0131663
Name: hsa_circ_MAPK14
Synonym: circMAPK14
Host Gene: MAPK14
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC specimens; adjacent non-tumour tissues

Cell lines:

SW480; DLD-1; LoVo; HT29; HCT116; Caco2; NCM460; HEK293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
DN
Associated gene: MKK6, MAPK14, FOXC1, U2AF2
Associated microRNA: -
Biological function: Suppresses proliferation, migration/invasion and metastasis; promotes apoptosis in colorectal cancer.
Molecular mechanism: circMAPK14 encodes a 175-aa peptide (circMAPK14-175aa) that competitively binds MKK6, represses MAPK14 phosphorylation and nuclear translocation, thereby promoting ubiquitin-proteasome-mediated degradation of FOXC1; FOXC1 represses U2AF2 transcription to reduce circMAPK14 biogenesis, forming a positive feedback loop.
Biological pathway or process:

MAPK (inhibits); proliferation (inhibits); apoptosis (promotes); migration (inhibits); invasion (inhibits); metastasis (inhibits); ubiquitination (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; ChIP / ChIP-seq; In Vivo Animal Model; H&E Staining; IF (Immunofluorescence); Bioinformatics Analysis

Clinical significance:

Lower circMAPK14 abundance was associated with tumour size, TNM stage and lymphatic metastasis, and patients with lower circMAPK14 had worse overall survival.

Description:

circMAPK14 (hsa_circ_0131663) is downregulated in colorectal cancer and mainly localized in the cytoplasm. It suppresses CRC proliferation and metastasis by encoding the peptide circMAPK14-175aa, which competes with MAPK14 for MKK6 binding, inhibits MAPK14 nuclear translocation, and promotes FOXC1 ubiquitination/degradation within a FOXC1-U2AF2 positive feedback loop.

Confidence score:

0.8192

Other information
Title:

A novel tumour suppressor protein encoded by circMAPK14 inhibits progression and metastasis of colorectal cancer by competitively binding to MKK6.

Journal: Clinical and translational medicine
Published: 2021
PubMed ID: 34709743
Study type:

combined biological and clinical study

Data availability: -
Code availability: -