| Expression pattern: |
UP |
| Associated gene: |
PGK1 |
| Associated microRNA: |
miR-335-5p |
| Biological function: |
Promotes BC cell viability, proliferation, invasion, tube angiogenesis, glycolysis and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
circ_0022587 acts as a molecular sponge for miR-335-5p and positively regulates PGK1 expression through the miR-335-5p/PGK1 axis. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); angiogenesis (promotes); glycolysis (promotes); apoptosis (inhibits); ceRNA regulation (promotes); cell cycle (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; MTT; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0022587 expression was associated with poor prognosis of BC patients and low survival rate; the study suggests potential therapeutic relevance for BC. |
| Description: |
circ_0022587 is upregulated in breast cancer tissues and cells, and high expression is associated with poor patient prognosis. Functionally, circ_0022587 promotes BC cell viability, proliferation, invasion, angiogenesis, glycolysis and in vivo tumor growth while suppressing apoptosis. Mechanistically, it acts through the circ_0022587/miR-335-5p/PGK1 regulatory axis. |
| Confidence score: |
0.8202 |