| Expression pattern: |
DN |
| Associated gene: |
KDM6A, SOX9, Ago2 |
| Associated microRNA: |
miR-758-3p |
| Biological function: |
promote hADSC chondrogenesis; maintain cartilage homeostasis; protect against chondrocyte degradation; alleviate OA progression |
| Molecular mechanism: |
circNFIX acts as a miRNA sponge for miR-758-3p to regulate KDM6A, affecting SOX9 methylation and ECM metabolism |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (not specified); apoptosis (not specified); migration (not specified); invasion (not specified); EMT (not specified) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; FISH / smFISH; ISH (In Situ Hybridization); Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining |
| Clinical significance: |
- |
| Description: |
circNFIX is decreased in OA cartilage and supports cartilage homeostasis by promoting chondrogenesis and reducing ECM catabolism. It mainly functions as a cytoplasmic ceRNA, sponging miR-758-3p to relieve repression of KDM6A, which affects SOX9 methylation and chondrocyte phenotype. Intra-articular overexpression of circNFIX alleviates OA progression in the DMM mouse model. |
| Confidence score: |
0.8398 |