circRNA basic information
circBase ID: hsa_circ_0003176
Name: hsa_circ_TBX5
Synonym: circ_0003176
Host Gene: TBX5
Genomic location(hg19): chr12:114823280-114841741:-
Genomic location(hg38): chr12:114385475-114403936:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; adjacent nontumor tissues; lung tissues
Cell lines:

H1299; A549; 95-D; H446; 16HBE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: RBM5
Associated microRNA: miR-182-5p
Biological function: Suppresses proliferation, migration, invasion, tumor growth and metastasis in NSCLC.
Molecular mechanism: Acts as a miRNA sponge (ceRNA) for miR-182-5p to upregulate RBM5.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; divergent primers PCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); ROC Analysis; Clinical Sample Validation
Clinical significance:

ROC curve indicated diagnostic value (AUC 98%).
Description:

In NSCLC, hsa_circ_0003176 is down-regulated and functions as a tumor suppressor by inhibiting proliferation, migration, invasion, and in vivo tumor growth/metastasis. Mechanistically, it sponges miR-182-5p to relieve repression of RBM5 (circRNA/miRNA/mRNA axis), and shows potential diagnostic value by ROC analysis.
Confidence score:

0.8683
Other information
Title:

hsa_circ_0003176 Suppresses the Progression of Non-Small-Cell Lung Cancer via Regulating miR-182-5p/RBM5 Axis.

Journal: Disease markers
Published: 2022
PubMed ID: 36193508
Study type:

combined biological and clinical study
Data availability: GSE101684; GSE112214
Code availability: -