| Expression pattern: |
UP |
| Associated gene: |
SLC1A5, Ago2 |
| Associated microRNA: |
miR-1827 |
| Biological function: |
promotes NSCLC cell proliferation, migration, and invasion; inhibits apoptosis; promotes tumor growth in vivo; promotes glutamine metabolism |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-1827 to positively regulate SLC1A5, thereby enhancing glutamine metabolism and NSCLC progression. |
| Biological pathway or process: |
glutaminolysis (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
circ_0000808 expression was associated with the tumor size, TNM stage, and lymph node metastasis in patients with NSCLC |
| Description: |
circ_0000808 is upregulated in NSCLC and promotes malignant phenotypes (proliferation, migration/invasion, tumor growth) while inhibiting apoptosis. Mechanistically, it functions as a ceRNA by sponging miR-1827 to upregulate SLC1A5, thereby enhancing glutamine metabolism in NSCLC cells. |
| Confidence score: |
0.8299 |