| Expression pattern: |
DN |
| Associated gene: |
RBM24, SMARCA5, TIMP3, SRSF1 |
| Associated microRNA: |
miR-670-5p |
| Biological function: |
circsSMARCA5 suppresses NSCLC progression by inhibiting tumor growth, reducing cell viability/proliferation/colony formation, and promoting apoptosis. |
| Molecular mechanism: |
circsSMARCA5 functions as a miR-670-5p sponge and positively regulates RBM24, thereby suppressing NSCLC progression through the miR-670-5p/RBM24 axis. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); apoptosis (promotes); other pathway/process (inhibits) |
| Detected method: |
Q
H
|
| Validation methods: |
divergent primers PCR; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; BrdU; Colony Formation Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Low circsSMARCA5 expression is associated with TNM staging, tumor size, and poor overall survival; circsSMARCA5 may have potential as a biomarker or therapeutic target for NSCLC. |
| Description: |
circsSMARCA5 is down-regulated in NSCLC tissues and cell lines, and low expression is associated with larger tumor size, advanced TNM stage, and poor overall survival. Functionally, circsSMARCA5 suppresses NSCLC tumor growth and cell proliferation while promoting apoptosis. Mechanistically, it acts as a sponge for miR-670-5p and upregulates RBM24, forming a circsSMARCA5/miR-670-5p/RBM24 suppressive axis. |
| Confidence score: |
0.8069 |