| Expression pattern: |
DS |
| Associated gene: |
- |
| Associated microRNA: |
miR-7a/b-5p, miR-450b-3p, miR-7685-3p, miR-7074-5p, miR-7-5p |
| Biological function: |
promotes phenotypic switching/polarization toward an anti-inflammatory macrophage phenotype; increases anti-inflammatory markers (e.g., CD206) and decreases pro-inflammatory marker CD86 when overexpressed; opposite effects upon knockdown |
| Molecular mechanism: |
predicted ceRNA/miRNA sponge interactions (circRNA-miRNA-mRNA network), including miR-7 family |
| Biological pathway or process: |
immune regulation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; divergent primers PCR; RNase R Treatment; Sanger Sequencing; Transfection; Flow Cytometry（Non-apoptosis/cycle）; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
In murine bone marrow-derived macrophages, circ-Cdr1as is decreased in pro-inflammatory (M1-like) polarization and increased in anti-inflammatory (M2-like) polarization. Overexpression promotes anti-inflammatory marker expression and CD206+ cells, while knockdown shifts cells toward a pro-inflammatory phenotype (increased CD86+), and bioinformatic predictions suggest circ-Cdr1as may act through a circRNA-miRNA-mRNA network including miR-7 family. |
| Confidence score: |
0.6475 |