circRNA basic information
circBase ID: -
Name: hsa_circ_CTNNB1
Synonym: circ‐CTNNB1
Host Gene: CTNNB1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

OS tissues; adjacent non‐tumour tissues
Cell lines:

143B; HOS; MG‐63; SJSA‐1; Saos‐2; U2OS; hFOB 1.19
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: RBM15, HK2, GPI, PGK1
Associated microRNA: -
Biological function: Promotes aerobic glycolysis, growth, invasion, and metastasis of osteosarcoma cells; drives osteosarcoma progression.
Molecular mechanism: Circ-CTNNB1 binds the m6A writer RBM15 and enhances RBM15-mediated m6A modification at the 3′-UTR of glycolysis genes (HK2, GPI, PGK1), stabilizing their mRNAs and increasing expression to promote glycolysis and tumor progression.
Biological pathway or process:

glycolysis (promotes); proliferation (promotes); invasion (promotes); metastasis (promotes); m6A modification (promotes)
Detected method:
Q
B
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; MeRIP / MeRIP-seq; Actinomycin D / DRB Stability Assay; Transfection; MTT; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; Survival Analysis; Western Blot; IF (Immunofluorescence)
Clinical significance:

Upregulated circ‐CTNNB1 was associated with lung metastasis in OS patients.
Description:

Circ-CTNNB1 is up-regulated in osteosarcoma and predominantly nuclear. It promotes OS cell growth, invasion and metastasis by binding the m6A writer RBM15 to enhance m6A modification and stabilize/activate glycolysis genes (HK2, GPI, PGK1), thereby driving aerobic glycolysis and tumor progression.
Confidence score:

0.8776
Other information
Title:

Circ-CTNNB1 drives aerobic glycolysis and osteosarcoma progression via m6A modification through interacting with RBM15.

Journal: Cell proliferation
Published: 2023
PubMed ID: 36181462
Study type:

combined biological and clinical study
Data availability: -
Code availability: -