| Expression pattern: |
UP |
| Associated gene: |
ODC1, FOXA1, Ago2 |
| Associated microRNA: |
miR-607 |
| Biological function: |
CircODC1 promotes HPV-positive cervical cancer cell proliferation, migration and invasion, and inhibits apoptosis. |
| Molecular mechanism: |
FOXA1 transcriptionally activates circODC1; circODC1 acts as a ceRNA that sponges miR-607 to up-regulate ODC1 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
These findings offer new insights into therapeutic strategies for HPV-positive CC patients. |
| Description: |
CircODC1 (hsa_circ_0052602) is up-regulated in HPV-positive cervical cancer cell lines and functions as an oncogenic circRNA. FOXA1 activates circODC1 transcription, and circODC1 promotes malignant cell growth by sponging miR-607 to relieve repression of its host gene ODC1. |
| Confidence score: |
0.8164 |