circRNA basic information
circBase ID: hsa_circ_0000735
Name: hsa_circ_P2RX1
Synonym: Circ_0000735
Host Gene: P2RX1
Genomic location(hg19): chr17:3802927-3808661:-
Genomic location(hg38): chr17:3899633-3905367:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tumor tissues; adjacent normal tissues; xenograft tumor tissues
Cell lines:

H1581; A549; H1299; 16HBE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: FAM83F, HK2, LDHA
Associated microRNA: miR-635
Biological function: promotes NSCLC cell proliferation, migration, invasion, glycolysis and tumor growth; inhibits apoptosis
Molecular mechanism: circ_0000735 acts as a miR-635 sponge and positively regulates FAM83F through the miR-635/FAM83F axis
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); glycolysis (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

circ_0000735 might be a promising biomarker for NSCLC
Description:

circ_0000735 is up-regulated in NSCLC tissues and cell lines. It promotes NSCLC proliferation, migration, invasion, glycolysis and tumor growth while suppressing apoptosis, mainly by sponging miR-635 to positively regulate FAM83F. The study suggests circ_0000735 may serve as a promising biomarker and therapeutic target for NSCLC.
Confidence score:

0.7799
Other information
Title:

Circ_0000735 enhances the proliferation, metastasis and glycolysis of non-small cell lung cancer by regulating the miR-635/FAM83F axis.

Journal: Experimental lung research
Published: 2021
PubMed ID: 33560141
Study type:

combined biological and clinical study
Data availability: The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -