| Expression pattern: |
UP |
| Associated gene: |
ANLN |
| Associated microRNA: |
miR-217 |
| Biological function: |
Promotes gastric cancer cell proliferation, migration, invasion and EMT; its knockdown suppresses these malignant phenotypes. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-217, relieving miR-217-mediated repression of ANLN; affects EMT marker proteins (E-cadherin, N-cadherin, Vimentin). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Transfection; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; Clinical Sample Validation |
| Clinical significance: |
Potential biomarker and therapeutic target for treatment of GC. |
| Description: |
circ_0000144 is up-regulated in gastric cancer tissues and cell lines and acts as an oncogenic circRNA. It promotes proliferation, migration, invasion and EMT by sponging miR-217, thereby relieving suppression of the downstream target ANLN. |
| Confidence score: |
0.7032 |