circRNA basic information
circBase ID: hsa_circ_0000285
Name: -
Synonym: circRNA_0000285
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0002974
MONDO name: cervical cancer
Disease details: Cervical cancer / CC
Disease DO ID:
 4362
Disease MeSH ID:
 -
Disease NCIt ID:
 C9311
Disease ICD11 ID:
 1256072522
Disease OMIM ID:
 603956
Species: Human
Species details: Homo sapiens
Tissue specimen:

CC tissues; adjacent normal tissues
Cell lines:

SiHa; HeLa; NC104; 293 T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: -
Associated microRNA: miR-654-3p
Biological function: Knockdown of circRNA_0000285 suppresses cell viability and promotes apoptosis in cervical cancer cells.
Molecular mechanism: circRNA_0000285 acts as a sponge for miR-654-3p; miR-654-3p inhibitor reverses phenotypes caused by circRNA_0000285 knockdown.
Biological pathway or process:

apoptosis (inhibits); proliferation (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Luciferase Reporter Assay; RNA Pull-Down; Transfection; MTT; Annexin V/PI Flow Cytometry; Western Blot; Clinical Sample Validation
Clinical significance:

-
Description:

In cervical cancer, circRNA_0000285 is up-regulated and directly binds (sponges) miR-654-3p. Silencing circRNA_0000285 reduces cervical cancer cell viability and increases apoptosis, accompanied by decreased Bcl-2 and increased Bax, and these effects are reversed by miR-654-3p inhibition.
Confidence score:

0.6772
Other information
Title:

circRNA_0000285 knockdown suppresses viability and promotes apoptosis of cervical cancer cells by sponging microRNA-654-3p.

Journal: Bioengineered
Published: 2022
PubMed ID: 35166172
Study type:

combined biological and clinical study
Data availability: -
Code availability: -