| Expression pattern: |
UP |
| Associated gene: |
KLF12, MMP-2, MMP-9, VEGFA |
| Associated microRNA: |
miR-577 |
| Biological function: |
circ_0007334 promotes CRC cell viability, colony formation, migration, invasion, angiogenesis, tumor growth and metastasis; circ_0007334 knockdown suppresses these malignant phenotypes in vitro and in vivo. |
| Molecular mechanism: |
circ_0007334 acts as a miR-577 sponge to derepress and increase KLF12 expression; exosomal circ_0007334 also contributes to CRC tumor growth and angiogenesis. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); angiogenesis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Tube Formation Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_0007334 may be an underlying tumor marker and therapeutic target for CRC. |
| Description: |
circ_0007334 is upregulated in CRC tissues, CRC cell lines and CRC cell-derived exosomes. It promotes CRC progression by sponging miR-577 and increasing KLF12 expression, thereby enhancing cell growth, migration, invasion, metastasis and angiogenesis. Knockdown of cellular or exosomal circ_0007334 suppresses tumor growth and angiogenesis in vivo, supporting its potential as a therapeutic target. |
| Confidence score: |
0.8443 |