| Expression pattern: |
UP |
| Associated gene: |
MAFB |
| Associated microRNA: |
miR-1286 |
| Biological function: |
promotes OS cell proliferation, migration, invasion and EMT; inhibits apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
circ_0051079 acts as a miRNA sponge for miR-1286 to upregulate MAFB (ceRNA-like regulation) |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Cohort Study |
| Clinical significance: |
High circ_0051079 expression was associated with tumor size and TNM stages, and patients with high circ_0051079 had lower survival rate. |
| Description: |
circ_0051079 is upregulated in osteosarcoma and mainly localized in the cytoplasm. It promotes malignant phenotypes (proliferation, migration/invasion, EMT) and tumor growth by sponging miR-1286, thereby relieving miR-1286-mediated repression of MAFB and increasing MAFB expression. |
| Confidence score: |
0.8427 |