| Expression pattern: |
UP |
| Associated gene: |
CDK6 |
| Associated microRNA: |
miR-106b |
| Biological function: |
Promotes CRC cell proliferation, migration, invasion, cell cycle progression, and tumor growth/tumorigenesis in vivo. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-106b to upregulate CDK6 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Clinical Sample Validation; Transfection; Luciferase Reporter Assay; Western Blot; CCK8; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
High expression level of hsa_circ_000984 was significantly associated with advanced TNM stage (III+IV) in CRC. |
| Description: |
hsa_circ_000984 is up-regulated in colorectal cancer tissues and cell lines and is associated with advanced TNM stage. It promotes CRC proliferation, cell-cycle progression, migration/invasion and xenograft tumor growth by sponging miR-106b and increasing CDK6 expression via a ceRNA mechanism. |
| Confidence score: |
0.7181 |