| Expression pattern: |
UP |
| Associated gene: |
IFIT1, STAT1, EIF4A3, STING, AGO2 |
| Associated microRNA: |
miR-576-3p |
| Biological function: |
promotes EC injury in vitro and atherosclerosis progression/lesion formation in vivo; promotes inflammatory response and impairs angiogenesis-related functions in HUVECs under ox-LDL |
| Molecular mechanism: |
ceRNA sponging miR-576-3p to increase IFIT1 and STAT1; STAT1 transcriptionally upregulates circ_0086296 (feedback loop); EIF4A3 binds flanking sequences to promote circ_0086296 biogenesis; EV/exosome-mediated transfer |
| Biological pathway or process: |
inflammation (promotes); ceRNA regulation (promotes); NF-kappaB (promotes); STING/IFN (promotes); migration (other); angiogenesis (inhibits) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; FISH / smFISH; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; Transwell Assay; Wound Healing Assay; Tube Formation Assay; Western Blot; ChIP / ChIP-seq; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); Oil Red O Staining; H&E Staining; ELISA; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
circ_0086296 was overexpressed in exosomes/serum EVs from patients with atherosclerosis (potential biomarker implication) |
| Description: |
circ_0086296 is up-regulated in atherosclerosis-related samples and promotes endothelial injury and atherosclerotic lesion formation. Mechanistically, it sponges miR-576-3p to increase IFIT1 and STAT1, while STAT1 transcriptionally enhances circ_0086296, forming a positive feedback loop; EIF4A3 promotes its biogenesis and it is enriched in patient serum EVs/exosomes. |
| Confidence score: |
0.8861 |