| Expression pattern: |
UP |
| Associated gene: |
Hu antigen R (HuR), FNDC3B, TGF-beta, TGFbetaRII, SMAD2/3, p-SMAD2/3 |
| Associated microRNA: |
- |
| Biological function: |
circFNDC3B is increased in active CD and regulates monocyte-macrophage function; circFNDC3B knockdown promotes FNDC3B expression, M2 macrophage activation, monocyte adhesion, and transmigration, while no significant effects were observed on proliferation, apoptosis, or phagocytosis. |
| Molecular mechanism: |
circFNDC3B competitively binds HuR and prevents HuR binding to FNDC3B mRNA, thereby lowering FNDC3B translation and regulating the FNDC3B/TGFbeta signaling axis during M2 macrophage activation. |
| Biological pathway or process: |
TGF-beta/SMAD (other); macrophage polarization (other); immune regulation (other); inflammation (other); migration (other); other pathway/process (other) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circFNDC3B expression is positively correlated with CD disease activity and severity indices, including CDAI, SES-CD, and CRP; it may serve as a potential therapeutic target for IBD. |
| Description: |
circFNDC3B is a human FNDC3B-derived circRNA upregulated in peripheral blood monocytes and inflamed mucosa of active Crohn's disease. It is mainly nuclear and regulates monocyte-macrophage behavior through a HuR/FNDC3B/TGFbeta mechanism; circFNDC3B knockdown promotes FNDC3B expression, M2 macrophage polarization, adhesion, and transmigration. Clinically, higher circFNDC3B levels correlate positively with CD activity and inflammation indices. |
| Confidence score: |
0.8237 |