circRNA basic information
circBase ID: -
Name: hsa_circ_PRMT5
Synonym: circRNA_101320 / circPRMT5
Host Gene: PRMT5
Genomic location(hg19): chr14:23395341-23396023:.
Genomic location(hg38): chr14:22926132-22926814:.
Subcellular localization: cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004986
MONDO name: urinary bladder carcinoma
Disease details: bladder cancer / BCa
Disease DO ID:
 4007
Disease MeSH ID:
 -
Disease NCIt ID:
 C4912
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

UCB tissues; matched adjacent normal bladder tissues; serum exosomes; urine exosomes; lung metastatic lesions
Cell lines:

T24; TCC-SUP; 5637; UM-UC-3; BIU; SV-HUC-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: AGO2, RISC complex, SNAIL1, E-cadherin, vimentin, N-cadherin
Associated microRNA: miR-30c
Biological function: Promotes UCB cell EMT, migration, invasion, aggressiveness and lung metastasis; high expression is associated with lymph node metastasis, advanced T and N status, advanced tumor progression and poorer survival.
Molecular mechanism: circPRMT5 acts as a miR-30c sponge in the cytoplasm, blocks the tumor-suppressive activity of miR-30c, and regulates the SNAIL1/E-cadherin EMT pathway.
Biological pathway or process:

ceRNA regulation (promotes); EMT (promotes); migration (promotes); invasion (promotes); metastasis (promotes)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; Northern Blot; FISH / smFISH; IHC (Immunohistochemistry); IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Upregulated circPRMT5 is positively associated with advanced clinical stage, advanced T and N status, lymph node metastasis, tumor progression, worse survival and poorer DFS; circPRMT5 in serum and urine exosomes predicts tumor lymph node metastasis and may serve as a prognostic biomarker and therapeutic target.
Description:

circPRMT5 is a PRMT5-derived human circRNA that is frequently upregulated in UCB tissues and in serum/urine exosomes from patients with UCB. It promotes EMT, migration, invasion and lung metastasis by sponging miR-30c and relieving miR-30c-mediated repression of the SNAIL1/E-cadherin pathway. Clinically, high circPRMT5 expression is associated with advanced disease, lymph node metastasis and poorer survival, supporting its value as a prognostic biomarker and potential therapeutic target.
Confidence score:

0.8988
Other information
Title:

PRMT5 Circular RNA Promotes Metastasis of Urothelial Carcinoma of the Bladder through Sponging miR-30c to Induce Epithelial-Mesenchymal Transition.

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research
Published: 2018
PubMed ID: 30305293
Study type:

combined biological and clinical study
Data availability: GSE112719; TCGA; Supplementary data at Clinical Cancer Research Online (http://clincancerres.aacrjournals.org/)
Code availability: -