| Expression pattern: |
UP |
| Associated gene: |
LDHA |
| Associated microRNA: |
miR-28-5p |
| Biological function: |
promotes cell proliferation, migration, invasion and glycolysis energy metabolism; inhibits apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circ-CSNK1G1 sponges miR-28-5p to positively regulate LDHA |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
high expression of circ-CSNK1G1 was associated with lower overall survival within 5 years |
| Description: |
circ-CSNK1G1 is up-regulated in TNBC tissues and cell lines and mainly localizes in the cytoplasm. It promotes TNBC malignant phenotypes (proliferation, migration, invasion, glycolysis) and tumor growth by sponging miR-28-5p to increase LDHA expression; higher circ-CSNK1G1 is associated with worse overall survival. |
| Confidence score: |
0.8069 |