| Expression pattern: |
UP |
| Associated gene: |
HNRNPC, EBF1 mRNA, SYT7, ELK1, Ago2 |
| Associated microRNA: |
miR-382-5p |
| Biological function: |
promotes CRC cell proliferation, migration, tumor growth and disease progression |
| Molecular mechanism: |
circ_0022340 recruits HNRNPC to stabilize EBF1 mRNA and thereby activates SYT7; circ_0022340 also acts as a ceRNA by targeting miR-382-5p to up-regulate ELK1 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); ceRNA regulation (promotes); mRNA stability (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; EdU Staining; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
might provide a novel target for CRC treatment |
| Description: |
circ_0022340 is up-regulated in colorectal cancer cells and acts as an oncogenic circRNA. It promotes CRC proliferation, migration and tumor growth by recruiting HNRNPC to stabilize EBF1 mRNA and activate SYT7, and by sponging miR-382-5p to increase ELK1 expression. The study suggests circ_0022340 may be a potential therapeutic target for CRC. |
| Confidence score: |
0.8597 |