| Expression pattern: |
UP |
| Associated gene: |
circPTPRM-187aa, IQGAP1, RAC1, CDC42 |
| Associated microRNA: |
- |
| Biological function: |
Promotes PTC cell proliferation, migration, invasion, EMT-related changes, tumor growth, and aggressiveness through its translated polypeptide circPTPRM-187aa. |
| Molecular mechanism: |
circPTPRM encodes the functional polypeptide circPTPRM-187aa, which binds IQGAP1, reduces IQGAP1 degradation, up-regulates RAC1 and CDC42, and activates the TGF-beta signaling pathway. |
| Biological pathway or process: |
TGF-beta/SMAD (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ubiquitination (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
S
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; FISH / smFISH; IF (Immunofluorescence); Clinical Sample Validation; Co-IP; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
High circPTPRM expression is positively correlated with tumor diameter >2 cm in PTC patients. |
| Description: |
circPTPRM is up-regulated in papillary thyroid carcinoma and is associated with larger tumor diameter. It promotes PTC proliferation, migration, invasion, EMT-related changes and xenograft tumor growth by encoding circPTPRM-187aa, which binds IQGAP1, reduces IQGAP1 degradation, and up-regulates RAC1 and CDC42 in the TGF-beta signaling pathway. |
| Confidence score: |
0.8071 |