| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
hsa-let-7c-3p, hsa-miR-214, hsa-miR-195 |
| Biological function: |
Potential oncogenic properties / associated with adrenal tumor formation (benign and malignant) based on overexpression in tumorous tissues versus normal adrenal cortex. |
| Molecular mechanism: |
Predicted miRNA sponging (in silico), but miRNA expression results not fully compatible with the sponging hypothesis. |
| Biological pathway or process: |
ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; divergent primers PCR; RNase R Treatment; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
Potential biomarker to distinguish adrenal tumors from normal adrenal cortex (validated differential expression between tumors and NAC). |
| Description: |
circPHC3 is significantly up-regulated in adrenocortical carcinoma and adenoma compared with normal adrenal cortex in FFPE clinical samples, confirmed by RT-qPCR after NGS discovery. The study explored a predicted miRNA-sponge role (let-7c-3p/miR-214/miR-195), but miRNA expression patterns were not fully consistent with the sponging hypothesis. |
| Confidence score: |
0.585 |