| Expression pattern: |
DN |
| Associated gene: |
ITCH, Wnt3a, beta-catenin, Dvl2, c-myc, cyclinD1 |
| Associated microRNA: |
miR-7, miR-20a, miR-214 |
| Biological function: |
Inhibits CRC cell proliferation and suppresses canonical Wnt/beta-catenin signaling. |
| Molecular mechanism: |
Acts as an miRNA sponge affecting ITCH levels and thereby inhibits Wnt/beta-catenin signaling (with downstream suppression of c-myc and cyclinD1). |
| Biological pathway or process: |
Wnt/beta-catenin (inhibits); proliferation (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
divergent primers PCR; RT-qPCR; RNase R Treatment; Sanger Sequencing; Luciferase Reporter Assay; Actinomycin D / DRB Stability Assay; Transfection; CCK8; Western Blot; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
In colorectal cancer, cir-ITCH is down-regulated and functions as a tumor-suppressive circRNA. It acts as an miRNA sponge to modulate ITCH, leading to inhibition of Wnt/beta-catenin signaling and reduced expression of downstream targets (e.g., c-myc and cyclinD1), thereby suppressing CRC cell proliferation. |
| Confidence score: |
0.7769 |