| Expression pattern: |
DN |
| Associated gene: |
Smad4, ADAR1, p21, Ago2 |
| Associated microRNA: |
hsa-miR-224 |
| Biological function: |
inhibits proliferation, invasion and migration; suppresses tumor growth and metastasis |
| Molecular mechanism: |
acts as a molecular sponge for miR-224 to upregulate p21 and Smad4; forms a negative regulatory loop with miR-224/Smad4/ADAR1, where ADAR1 inhibits hsa_circ_0004872 biogenesis and Smad4 represses ADAR1 transcription |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Clinical Sample Validation; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Northern Blot; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; EdU Staining; CCK8; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); ChIP / ChIP-seq; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
The expression level of hsa_circ_0004872 was associated with tumor size and local lymph node metastasis. |
| Description: |
hsa_circ_0004872 (circMAPK1) is down-regulated in gastric cancer and functions as a tumor suppressor. It mainly localizes to the cytoplasm and sponges miR-224, thereby increasing p21 and Smad4 expression and inhibiting proliferation, invasion/migration, and metastasis. The study proposes a negative feedback circuit in which ADAR1 suppresses hsa_circ_0004872, while Smad4 represses ADAR1 transcription, collectively modulating the hsa_circ_0004872/miR-224 axis. |
| Confidence score: |
0.886 |