| Expression pattern: |
UP |
| Associated gene: |
AGO2, BPTF, c-myc, HIF1A, BIN1, UBE2O, RNA polymerase II |
| Associated microRNA: |
miR-628-3p |
| Biological function: |
Promotes pancreatic cancer cell proliferation, migration/metastasis, and glycolysis; promotes tumor growth in vivo. |
| Molecular mechanism: |
Hypoxia/HIF1A transcriptionally activates host gene PDK1 leading to increased circPDK1; circPDK1 sponges miR-628-3p to release BPTF and activate c-myc; circPDK1 also acts as a scaffold enhancing UBE2O-BIN1 interaction to promote ubiquitination-mediated degradation of BIN1, further activating c-myc and glycolysis. |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); migration (promotes); metastasis (promotes); ceRNA regulation (promotes); ubiquitination (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; ISH (In Situ Hybridization); Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; ChIP / ChIP-seq; Co-IP; Western Blot; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
High circPDK1 expression is associated with advanced pathological stage, lymph node metastasis, and poor prognosis; circPDK1 is abundant in serum exosomes of PC patients but almost absent in healthy controls, suggesting biomarker potential for diagnosis and prognosis. |
| Description: |
circPDK1 is upregulated in pancreatic cancer tissues and serum exosomes and correlates with poor prognosis. Under hypoxia, HIF1A increases circPDK1 via activating the PDK1 promoter. circPDK1 promotes proliferation, migration/metastasis and glycolysis by activating c-myc through a miR-628-3p/BPTF ceRNA axis and by scaffolding UBE2O-mediated ubiquitination/degradation of BIN1. |
| Confidence score: |
0.8995 |