| Expression pattern: |
UP |
| Associated gene: |
DDR1, JAG1 |
| Associated microRNA: |
miR-199b-5p |
| Biological function: |
Promotes migration, invasion and metastasis of colorectal cancer cells in vitro and in vivo; promotes CRC progression and liver metastasis; may be useful for CRC detection and prognosis prediction. |
| Molecular mechanism: |
circ-NSD2 functions as a miR-199b-5p sponge, thereby upregulating DDR1 and JAG1 and their downstream signalling to promote CRC migration, invasion and metastasis. |
| Biological pathway or process: |
ceRNA regulation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ-NSD2 was elevated in CRC tissues, advanced stages and metastatic tumours, and may serve as a prognostic factor, prognosis predictor and therapeutic target for antimetastatic therapy in CRC patients. |
| Description: |
This study identifies circ-NSD2 as an up-regulated, conserved circular RNA in colorectal cancer and CRC metastasis. circ-NSD2 mainly localizes to the cytoplasm and promotes CRC cell migration, invasion and metastasis by sponging miR-199b-5p, thereby increasing DDR1 and JAG1 expression. Clinically, its elevated expression in CRC tissues, advanced stages and metastatic tumours suggests potential value as a prognostic marker and therapeutic target. |
| Confidence score: |
0.8448 |