| Expression pattern: |
UP |
| Associated gene: |
EPHB2, MMP-2, TIMP-1 |
| Associated microRNA: |
miR-4268 |
| Biological function: |
Promotes extracellular matrix degradation in AAA by increasing MMP-2 and reducing TIMP-1 in VSMCs. |
| Molecular mechanism: |
Acts as a miR-4268 sponge to upregulate EPHB2, thereby promoting ECM degradation (increased MMP-2 and decreased TIMP-1). |
| Biological pathway or process: |
ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; Western Blot; Clinical Sample Validation |
| Clinical significance: |
circRBM33 may be a potential diagnostic and therapeutic target for AAA. |
| Description: |
circRBM33 is up-regulated in abdominal aortic aneurysm tissues and Ang II-induced VSMCs. Functionally, circRBM33 overexpression promotes ECM degradation by increasing MMP-2 and decreasing TIMP-1, mechanistically via sponging miR-4268 to upregulate EPHB2 (circRBM33/miR-4268/EPHB2 axis). |
| Confidence score: |
0.7519 |