| Expression pattern: |
UP |
| Associated gene: |
c-Myc, c-MYC mRNA, GPC3 promoter |
| Associated microRNA: |
miR-526b |
| Biological function: |
Promotes proliferation of HCC cells; downregulation inhibits proliferation of HCC cells. |
| Molecular mechanism: |
hsa_circ_0091581 acts as a sponge of miR-526b, relieves miR-526b-mediated repression of c-Myc, and participates in a hsa_circ_0091581/miR-526b/c-Myc/hsa_circ_0091581 positive feedback loop; c-Myc upregulates hsa_circ_0091581 expression via the GPC3 promoter. |
| Biological pathway or process: |
proliferation (promotes); ceRNA regulation (promotes); cell cycle (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Luciferase Reporter Assay; Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High hsa_circ_0091581 expression was correlated with tumor size and associated with low disease-free survival and overall survival; it may be a potential prognostic biomarker and therapeutic target for HCC. |
| Description: |
hsa_circ_0091581 is upregulated in HCC tissues and cell lines, and high expression is associated with larger tumor size and worse DFS/OS. Functionally, it promotes HCC cell proliferation by sponging miR-526b and relieving repression of c-Myc, forming a positive feedback loop in which c-Myc also upregulates hsa_circ_0091581. The study suggests hsa_circ_0091581 as a potential prognostic biomarker and therapeutic target for HCC. |
| Confidence score: |
0.7968 |