circRNA basic information
circBase ID: hsa_circ_0007494
Name: hsa_circ_ROCK2
Synonym: -
Host Gene: ROCK2
Genomic location(hg19): chr2:11426664-11428575:-
Genomic location(hg38): chr2:11286538-11288449:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

PCa tissues; benign prostatic hyperplasia tissues; tumor xenograft tissues
Cell lines:

C4-2; PC3; 22Rv1; DU145; LNCaP; RWPE-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: PTEN, AGO2, ROCK2, Ki67
Associated microRNA: miR-616
Biological function: Suppresses PCa progression by inhibiting cell proliferation, migration, invasion, and tumor growth in vivo.
Molecular mechanism: Hsa_circ_0007494 acts as a molecular sponge for miR-616, thereby upregulating PTEN and suppressing PCa progression through the hsa_circ_0007494/miR-616/PTEN regulatory axis.
Biological pathway or process:

ceRNA regulation (other); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); PI3K/AKT/mTOR (inhibits)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; IHC (Immunohistochemistry); Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model
Clinical significance:

Low hsa_circ_0007494 expression was linked to metastasis and advanced tumor stage in PCa patients; hsa_circ_0007494 may be a treatment target for PCa.
Description:

Hsa_circ_0007494 is down-regulated in prostate cancer tissues and cell lines, and low expression is associated with metastasis and advanced tumor stage. Functionally, hsa_circ_0007494 acts as a tumor suppressor by inhibiting PCa cell proliferation, migration, invasion, and xenograft tumor growth. Mechanistically, it sponges miR-616 to upregulate PTEN, forming the hsa_circ_0007494/miR-616/PTEN axis.
Confidence score:

0.8032
Other information
Title:

Hsa_circ_0007494 suppresses prostate cancer progression via miR-616/PTEN axis.

Journal: Experimental cell research
Published: 2020
PubMed ID: 32810511
Study type:

combined biological and clinical study
Data availability: Research data are not shared; Supplementary data: https://doi.org/10.1016/j.yexcr.2020.112233
Code availability: -