circRNA basic information
circBase ID: hsa_circ_0096442
Name: -
Synonym: circ_0096442
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005061
MONDO name: lung adenocarcinoma
Disease details: lung adenocarcinoma / LUAD
Disease DO ID:
 3910
Disease MeSH ID:
 C538231
Disease NCIt ID:
 C3512
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

peripheral blood
Cell lines:

A549; HS-5
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DS
Associated gene: Caspase3, BAX, BCL2
Associated microRNA: hsa-miR-326
Biological function: promotes proliferation; promotes migration and invasion; inhibits apoptosis
Molecular mechanism: Acts as a miR-326 sponge (ceRNA-like interaction) validated by dual-luciferase assay and cytoplasmic co-localization.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Wound Healing Assay; Luciferase Reporter Assay; FISH / smFISH; Bioinformatics Analysis
Clinical significance:

circ_0096442 may be a biomarker for LUAD-BM.
Description:

In LUAD with bone metastasis, circ_0096442 is a markedly dysregulated circRNA detected in peripheral blood and validated by RT-qPCR. Functionally, it promotes A549 cell proliferation, migration and invasion while inhibiting apoptosis in a bone-microenvironment co-culture model, and it acts as a sponge for hsa-miR-326 with cytoplasmic co-localization and luciferase-validated binding.
Confidence score:

0.6975
Other information
Title:

Comprehensive circular RNA expression profile of lung adenocarcinoma with bone metastasis: Identification of potential biomarkers.

Journal: Frontiers in genetics
Published: 2022
PubMed ID: 36051693
Study type:

combined biological and clinical study
Data availability: SRA database PRJNA562624
Code availability: -