| Expression pattern: |
UP |
| Associated gene: |
ADAR1, AGO2, EIF4B, YRDC |
| Associated microRNA: |
miR-424-5p |
| Biological function: |
promotes proliferation, migration, invasion, tumorigenesis and bone destruction; inhibits apoptosis |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-424-5p to regulate EIF4B and YRDC; circRBMS3 upregulation is regulated by ADAR1 |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes) |
| Detected method: |
Q
B
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model; TUNEL; H&E Staining; Bioinformatics Analysis; ISH (In Situ Hybridization) |
| Clinical significance: |
CircRBMS3 might serve as a new biomarker regarding poor prognosis in OS. |
| Description: |
circRBMS3 (hsa_circ_0064644), derived from RBMS3 exons 7-10, is up-regulated in osteosarcoma and mainly localized in the cytoplasm. It promotes OS cell proliferation, migration/invasion and in vivo tumorigenesis/bone destruction by acting as a ceRNA that sponges miR-424-5p to upregulate EIF4B and YRDC; ADAR1 downregulation contributes to circRBMS3 overexpression. |
| Confidence score: |
0.8535 |