circRNA basic information
circBase ID: hsa_circ_0007255
Name: hsa_circ_KIF4A
Synonym: circKIF4A
Host Gene: KIF4A
Genomic location(hg19): chrX:69549254-69553539:+
Genomic location(hg38): chrX:70329404-70333689:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0021042
MONDO name: glioma
Disease details: glioma
Disease DO ID:
 -
Disease MeSH ID:
 D005910
Disease NCIt ID:
 C3059
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

glioma tissues; adjacent normal brain tissues
Cell lines:

A172; SHG-44; U251; T98G; BT325; HEB
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ALDOA
Associated microRNA: miR-335-5p
Biological function: promotes glioma growth, proliferation, invasion/migration, glycolysis, and temozolomide resistance; increases temozolomide resistance and decreases sensitivity to temozolomide
Molecular mechanism: ceRNA mechanism: circKIF4A binds/sponges miR-335-5p to upregulate ALDOA
Biological pathway or process:

glycolysis (promotes); proliferation (promotes); invasion (promotes); migration (promotes); chemoresistance (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis
Clinical significance:

-
Description:

circKIF4A is up-regulated in glioma tissues and cell lines and promotes tumor growth, proliferation, invasion/migration, glycolysis, and temozolomide resistance. Mechanistically, it mainly localizes in the cytoplasm and acts as a ceRNA by binding miR-335-5p to relieve repression of ALDOA, enhancing glycolysis and malignant phenotypes.
Confidence score:

0.8443
Other information
Title:

CircKIF4A promotes glioma growth and temozolomide resistance by accelerating glycolysis.

Journal: Cell death & disease
Published: 2022
PubMed ID: 36030248
Study type:

combined biological and clinical study
Data availability: The raw data of this paper will be available from the authors.
Code availability: -