| Expression pattern: |
UP |
| Associated gene: |
SOX13, Ago2 |
| Associated microRNA: |
miR-133b |
| Biological function: |
Hsa_circ_0071589 promotes CRC cell stemness, oxaliplatin resistance, tumor growth, metastatic nodules, migration, invasion and EMT, and suppresses apoptosis or oxaliplatin sensitivity. |
| Molecular mechanism: |
Hsa_circ_0071589 acts as a ceRNA by sponging miR-133b, thereby indirectly upregulating SOX13 expression to promote stemness and oxaliplatin resistance in CRC. |
| Biological pathway or process: |
ceRNA regulation (other); stemness (promotes); chemoresistance (promotes); apoptosis (inhibits); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
Hsa_circ_0071589 or miR-133b might serve as novel targets for drug resistance in CRC and may help improve chemotherapy sensitivity. |
| Description: |
Hsa_circ_0071589 is upregulated in colorectal cancer tissues and cells and is further elevated in oxaliplatin-resistant CRC cells. It promotes CRC stemness, tumor growth, metastasis-related phenotypes and oxaliplatin resistance by sponging miR-133b and indirectly increasing SOX13 expression, making it a potential therapeutic target for OXP-resistant CRC. |
| Confidence score: |
0.8005 |