| Expression pattern: |
UP |
| Associated gene: |
FOSL2 |
| Associated microRNA: |
miR-619-5p |
| Biological function: |
Promotes CRC cell proliferation, migration, invasion, angiogenesis, and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
circ-FAT1 acts as a miR-619-5p sponge to up-regulate FOSL2 expression, forming a circ-FAT1/miR-619-5p/FOSL2 regulatory axis in CRC. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); angiogenesis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; Transwell Assay; Tube Formation Assay; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
Potential therapeutic target or approach for CRC targeted therapy. |
| Description: |
circ-FAT1 is up-regulated in colorectal cancer tissues and cells and functions as a tumor-promoting circRNA. Mechanistically, cytoplasmic circ-FAT1 sponges miR-619-5p to relieve repression of FOSL2, thereby promoting CRC cell proliferation, migration, invasion, angiogenesis, and xenograft tumor growth while suppressing apoptosis. The study suggests circ-FAT1 as a potential therapeutic target in CRC. |
| Confidence score: |
0.8175 |