| Expression pattern: |
DN |
| Associated gene: |
BAK1, Ago2 |
| Associated microRNA: |
miR-331-3p |
| Biological function: |
Circ-0051443 suppresses malignant biological behaviors of HCC cells, inhibits proliferation and tumor growth, promotes apoptosis, and arrests the cell cycle in G0/G1 phase. |
| Molecular mechanism: |
Exosomal circ-0051443 functions as a ceRNA by sponging miR-331-3p to regulate BAK1 expression and suppress HCC progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (inhibits); apoptosis (promotes); cell cycle (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
S
M
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Microarray; Clinical Sample Validation; ROC Analysis; Transfection; CCK8; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Bioinformatics Analysis; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
Exosomal circ-0051443 can distinguish patients with HCC from controls and may serve as a diagnostic marker, predictor, and potential therapeutic target for HCC. |
| Description: |
hsa_circ_0051443/circ-0051443 is down-regulated in HCC tissues and plasma exosomes. Normal liver cell-derived exosomes can transmit circ-0051443 to HCC cells, where it suppresses proliferation and tumor growth, promotes apoptosis, and arrests the cell cycle. Mechanistically, exosomal circ-0051443 acts as a ceRNA for miR-331-3p to upregulate BAK1 and may serve as a diagnostic biomarker and therapeutic target for HCC. |
| Confidence score: |
0.8423 |