circRNA basic information
circBase ID: hsa_circ_0070659
Name: hsa_circ_CCDC109B
Synonym: circ_0070659
Host Gene: CCDC109B
Genomic location(hg19): chr4:110580166-110581521:+
Genomic location(hg38): chr4:109659010-109660365:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; matched adjoining normal tissues / paired non-tumor tissues / adjacent normal tissues
Cell lines:

A549; PC9; H460; H82; H1650; H1299; 16HBE; HEK-293 T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: RAB3C
Associated microRNA: miR-377
Biological function: promotes NSCLC cell proliferation, migration, and invasion
Molecular mechanism: Acts as a ceRNA by sponging miR-377 to relieve repression of RAB3C, thereby promoting malignant phenotypes in NSCLC.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; Cohort Study
Clinical significance:

High circ_0070659 expression shows diagnostic value (ROC AUC=0.9666) and is described as a diagnostic and prognostic marker for NSCLC.
Description:

circ_0070659 (hsa_circ_0070659), derived from CCDC109B, is up-regulated in NSCLC tissues and cell lines and shows strong diagnostic performance by ROC analysis. It promotes NSCLC proliferation, migration, and invasion by sponging miR-377 and thereby sustaining RAB3C expression (circ_0070659/miR-377/RAB3C ceRNA axis).
Confidence score:

0.8586
Other information
Title:

CircRNA hsa_circ_0070659 predicts poor prognosis and promotes non-small cell lung cancer (NSCLC) progression via microRNA-377 (miR-377) / Ras-Associated Binding Protein 3C (RAB3C) pathway.

Journal: Bioengineered
Published: 2022
PubMed ID: 36694911
Study type:

combined biological and clinical study
Data availability: GSE158695; https://doi.org/10.1080/21655979.2022.2091572
Code availability: -