| Expression pattern: |
DN |
| Associated gene: |
hnRNPF, YAP, SOX9, ISG15 |
| Associated microRNA: |
- |
| Biological function: |
circ_0057582 suppresses tumor growth, inhibits proliferation, migration and invasion, promotes apoptosis, and inhibits M2-type tumor-associated macrophage polarization in breast cancer. |
| Molecular mechanism: |
circ_0057582 binds to hnRNPF to negatively regulate YAP mRNA stability and reduce YAP/SOX9 signaling; it also binds ISG15 and promotes ISG15 protein degradation through autophagy, reducing secreted ISG15 and preventing macrophage M2 polarization. |
| Biological pathway or process: |
Hippo/YAP (inhibits); autophagy (promotes); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); immune regulation (promotes); macrophage polarization (inhibits); mRNA stability (inhibits); other pathway/process (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; Microarray; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; IHC (Immunohistochemistry); IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; In Vivo Animal Model; Western Blot; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ_0057582 expression correlated with pathological type and serum circ_0057582 showed diagnostic potential for discriminating breast cancer patients from healthy donors, with improved performance when combined with CEA and CA-153. |
| Description: |
circ_0057582 is a downregulated tumor-suppressive circRNA in breast cancer tissues, serum, and cell lines. It inhibits breast cancer growth, proliferation, migration, and invasion through binding hnRNPF and destabilizing YAP mRNA, thereby reducing SOX9. It also promotes autophagic degradation of ISG15 to reduce secreted ISG15 and suppress M2 polarization of tumor-associated macrophages, and shows potential as a serum diagnostic biomarker. |
| Confidence score: |
0.8933 |