circRNA basic information
circBase ID: hsa_circ_0000043
Name: hsa_circ_PUM1
Synonym: -
Host Gene: PUM1
Genomic location(hg19): chr1:31465236-31468067:-
Genomic location(hg38): chr1:30992389-30995220:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0011962
MONDO name: endometrial cancer
Disease details: endometrial cancer
Disease DO ID:
 1380
Disease MeSH ID:
 -
Disease NCIt ID:
 C27815
Disease ICD11 ID:
 -
Disease OMIM ID:
 608089
Species: Human
Species details: Homo sapiens
Tissue specimen:

endometrial carcinoma tissues; normal endometrial tissue
Cell lines:

HEC-1B; Ishikawa; hESC; EEC; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: NOTCH3
Associated microRNA: miR-136
Biological function: promotes proliferation, migration and invasion; inhibits apoptosis; promotes tumorigenicity in vivo
Molecular mechanism: Acts as a miRNA sponge for miR-136 to up-regulate NOTCH3 (ceRNA-like mechanism).
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes); Notch (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; MTT; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Luciferase Reporter Assay; Bioinformatics Analysis; Western Blot; In Vivo Animal Model
Clinical significance:

-
Description:

circ_PUM1 (circ_0000043) is up-regulated in endometrial cancer tissues and cell lines. It promotes malignant phenotypes (proliferation, migration, invasion) and inhibits apoptosis, and its knockdown suppresses xenograft tumor growth. Mechanistically, circ_PUM1 sponges miR-136 to relieve repression of NOTCH3, thereby activating Notch-related oncogenic effects.
Confidence score:

0.6542
Other information
Title:

Circ_PUM1 promotes the development of endometrial cancer by targeting the miR-136/NOTCH3 pathway.

Journal: Journal of cellular and molecular medicine
Published: 2020
PubMed ID: 32073729
Study type:

combined biological and clinical study
Data availability: The datasets used and/or analysed during the current study available from the corresponding author on reasonable request.
Code availability: -