| Expression pattern: |
UP |
| Associated gene: |
TGFBR2 |
| Associated microRNA: |
miR-370-3p |
| Biological function: |
promotes migration and invasion; promotes tumor growth |
| Molecular mechanism: |
ceRNA (miRNA sponge) of miR-370-3p to upregulate TGFBR2 |
| Biological pathway or process: |
migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RNase R Treatment; divergent primers PCR; Sanger Sequencing; RT-qPCR; FISH / smFISH; Transfection; Transwell Assay; RNA Pull-Down; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
may be a potential serum biomarker of GBM |
| Description: |
circARID1A is up-regulated in glioblastoma tissues and cell lines and promotes GBM migration/invasion. It acts mainly in the cytoplasm as a miRNA sponge for miR-370-3p, thereby increasing TGFBR2 expression and enhancing invasive phenotypes; knockdown reduces tumor growth in a U87 intracranial xenograft model. circARID1A is enriched in GBM cell-derived exosomes and may serve as a serum biomarker. |
| Confidence score: |
0.8609 |