circRNA basic information
circBase ID: hsa_circ_0022392
Name: hsa_circ_FADS2
Synonym: circRNA fatty acid desaturase 2 (circFADS2) / CircFADS2
Host Gene: -
Genomic location(hg19): chr11:61630443-61631258:+
Genomic location(hg38): chr11:61862971-61863786:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0002529
MONDO name: skin squamous cell carcinoma
Disease details: cutaneous squamous cell carcinoma / CSCC
Disease DO ID:
 3151
Disease MeSH ID:
 -
Disease NCIt ID:
 C4819
Disease ICD11 ID:
 1448983042
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

CSCC tissues; adjacent normal tissues; normal skin tissues; tumor tissues
Cell lines:

SCL-1; A-431; HaCaT
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: HOXA9, cyclinD1, c-myc, MMP9, HK2, Ago2
Associated microRNA: miR-766-3p
Biological function: CircFADS2 acts as a tumor-suppressing circRNA in CSCC; its overexpression inhibits cell cycle progression, proliferation, migration, invasion, metastasis, glycolysis, and tumor growth in vivo.
Molecular mechanism: CircFADS2 functions as a cytoplasmic ceRNA/miRNA sponge that binds miR-766-3p, thereby relieving miR-766-3p-mediated repression of HOXA9 and altering downstream proteins associated with cell cycle, metastasis, and glycolysis.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); glycolysis (inhibits); cell cycle (inhibits); ceRNA regulation (other)
Detected method:
Q
M
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; Nuclear-Cytoplasmic Fractionation; Survival Analysis; Bioinformatics Analysis
Clinical significance:

CircFADS2 downregulation was associated with lymphoid node metastasis and poor survival; circFADS2 may serve as a promising prognostic biomarker and therapeutic target for CSCC.
Description:

CircFADS2 is downregulated in CSCC tissues and cell lines and appears to function as a tumor suppressor. Mechanistically, cytoplasmic circFADS2 sponges miR-766-3p to promote HOXA9 expression, thereby inhibiting CSCC cell proliferation, migration, invasion, glycolysis, and xenograft tumor growth. Clinically, lower circFADS2 expression is associated with lymph node metastasis and poorer survival.
Confidence score:

0.8571
Other information
Title:

Circular RNA circFADS2 inhibits the progression of cutaneous squamous cell carcinoma by regulating miR-766-3p/HOXA9 axis.

Journal: Histology and histopathology
Published: 2022
PubMed ID: 34913472
Study type:

combined biological and clinical study
Data availability: GSE74758; Please contact the correspondence author for the data request.
Code availability: -