| Expression pattern: |
UP |
| Associated gene: |
NRAS, ERK, AGO2 |
| Associated microRNA: |
miR-27b-3p |
| Biological function: |
promotes proliferation, migration, invasion and tumor growth/metastasis; regulates tumor-associated macrophage infiltration |
| Molecular mechanism: |
ceRNA/miRNA sponge: circRanGAP1 sponges miR-27b-3p to relieve repression of NRAS, activating ERK phosphorylation (MAPK signaling) and promoting HCC progression; associated with TAM infiltration |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); MAPK (promotes); ERK (promotes); ceRNA regulation (promotes); macrophage polarization (other); immune regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
High circRanGAP1 is associated with enlarged tumors and advanced TNM stage; proposed as a potential biomarker in HCC. |
| Description: |
circRanGAP1 (hsa_circ_0063513), derived from RanGAP1, is up-regulated in HCC tissues and cell lines and promotes HCC proliferation, migration, invasion, and in vivo tumor growth/metastasis. It mainly localizes to the cytoplasm and functions as a miR-27b-3p sponge to upregulate NRAS and activate ERK/MAPK signaling, and is linked to increased tumor-associated macrophage infiltration. |
| Confidence score: |
0.8512 |