circRNA basic information
circBase ID: hsa_circ_0004194
Name: hsa_circ_CTNNB1
Synonym: circbeta-catenin
Host Gene: beta-catenin
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0002691
MONDO name: liver cancer
Disease details: liver cancer
Disease DO ID:
 3571
Disease MeSH ID:
 -
Disease NCIt ID:
 C34803
Disease ICD11 ID:
 1605020868
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; adjacent normal tissues; tumor tissues; lung tissues; liver tissues
Cell lines:

Huh7; HepG2; PLC/PRF/5; LO2; SW620; HEK293
In vivo animal model:

cell line-derived xenograft; syngeneic model
circRNA-disease information
Expression pattern:
UP
Associated gene: beta-catenin, beta-catenin-370aa, GSK3beta
Associated microRNA: -
Biological function: promotes liver cancer cell growth, cell cycle progression, migration, invasion, tumorigenesis and metastasis
Molecular mechanism: circbeta-catenin is translated to beta-catenin-370aa, which binds GSK3beta and antagonizes GSK3beta-induced phosphorylation/ubiquitination/degradation of full-length beta-catenin, activating Wnt/beta-catenin signaling
Biological pathway or process:

Wnt/beta-catenin (promotes); proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ubiquitination (inhibits)
Detected method:
Q
B
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Northern Blot; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; MTT; Colony Formation Assay; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; RNA-seq; Bioinformatics Analysis; Luciferase Reporter Assay; Co-IP; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry)
Clinical significance:

circbeta-catenin is significantly increased in liver cancer tissues compared with adjacent normal tissues
Description:

circbeta-catenin is up-regulated in HCC and promotes malignant phenotypes (proliferation, cell cycle progression, migration/invasion, tumor growth and metastasis). Mechanistically, it encodes beta-catenin-370aa, which binds GSK3beta and stabilizes full-length beta-catenin to activate Wnt/beta-catenin signaling.
Confidence score:

0.8661
Other information
Title:

Translation of the circular RNA circbeta-catenin promotes liver cancer cell growth through activation of the Wnt pathway.

Journal: Genome biology
Published: 2019
PubMed ID: 31027518
Study type:

combined biological and clinical study
Data availability: -
Code availability: -