| Expression pattern: |
UP |
| Associated gene: |
ENO1, NFKB1, NF-kappaB signaling pathway |
| Associated microRNA: |
- |
| Biological function: |
Promotes chondrocyte extracellular matrix (ECM) catabolism/degradation and aggravates OA progression. |
| Molecular mechanism: |
circNFKB1 binds ENO1, regulates its parental gene NFKB1 expression, and sustains NF-kappaB signaling activation. |
| Biological pathway or process: |
NF-kappaB (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; ISH (In Situ Hybridization); FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Actinomycin D / DRB Stability Assay; divergent primers PCR; Sanger Sequencing; RNA Pull-Down; Western Blot; IF (Immunofluorescence); Transfection; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
Suggested as a promising therapeutic target; potential stable biomarker (speculative). |
| Description: |
circNFKB1 (has_circ_0008012), derived from NFKB1 exons 2-5 and present in both nucleus and cytoplasm, is upregulated in inflamed chondrocytes and osteoarthritic cartilage. It promotes ECM catabolism and OA progression by binding ENO1, enhancing parental gene NFKB1 expression, and sustaining NF-kappaB signaling activation. |
| Confidence score: |
0.8193 |