circRNA basic information
circBase ID: hsa_circ_0007334
Name: hsa_circ_MBOAT2
Synonym: circMBOAT2
Host Gene: MBOAT2
Genomic location(hg19): chr2:9083315-9098771:-
Genomic location(hg38): chr2:8943186-8958642:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; adjacent normal tissues; serum
Cell lines:

FHC; HCT-8; DLD-1; SW480; HCT-116; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: Ago2, TROAP
Associated microRNA: miR-519d-3p
Biological function: promotes proliferation, migration, and invasion; promotes tumor growth in vivo
Molecular mechanism: Acts as a miRNA sponge of miR-519d-3p to regulate TROAP (ceRNA mechanism); binds Ago2
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; Sanger Sequencing; RNase R Treatment; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; Survival Analysis; ROC Analysis; Bioinformatics Analysis; Clinical Sample Validation
Clinical significance:

Diagnostic tumor marker in CRC (ROC AUC 0.750 in serum); independent prognostic marker for OS in CRC tissues
Description:

circMBOAT2 (hsa_circ_0007334), derived from MBOAT2, is up-regulated in colorectal cancer tissues and patient serum and is associated with tumor stage. It promotes CRC cell proliferation, migration, invasion and tumor growth by acting as a miRNA sponge for miR-519d-3p, thereby upregulating TROAP. It shows diagnostic value (ROC) and high expression predicts poor overall survival, serving as an independent prognostic marker.
Confidence score:

0.8766
Other information
Title:

Circular noncoding RNA circMBOAT2 is a novel tumor marker and regulates proliferation/migration by sponging miR-519d-3p in colorectal cancer.

Journal: Cell death & disease
Published: 2020
PubMed ID: 32796815
Study type:

combined biological and clinical study
Data availability: GSE77661
Code availability: -