| Expression pattern: |
DN |
| Associated gene: |
PTEN, QKI, AGO2 |
| Associated microRNA: |
miR-532-5p |
| Biological function: |
Suppresses osteosarcoma cell proliferation and migration; inhibits tumor growth and lung metastasis in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-532-5p, thereby upregulating PTEN; circRNA biogenesis partially regulated by QKI via binding flanking introns; associated with AGO2 complex. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (other); PI3K/AKT/mTOR (inhibits) |
| Detected method: |
Q
H
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Downregulated circROCK1-E3/E4 correlates with unfavorable clinical features and shorter overall survival; ROC AUC 0.9048 suggests diagnostic/prognostic biomarker potential. |
| Description: |
circROCK1-E3/E4 (hsa_circ_0108024), derived from ROCK1 exons 3-4, is down-regulated in osteosarcoma and functions as a tumor suppressor. It mainly localizes to the cytoplasm and inhibits proliferation, migration/invasion, tumor growth and lung metastasis by sponging miR-532-5p to increase PTEN; its biogenesis is partially promoted by QKI. |
| Confidence score: |
0.8711 |