| Expression pattern: |
UP |
| Associated gene: |
FZD4, beta-catenin, c-Myc, Cyclin D1 |
| Associated microRNA: |
miR-143-3p |
| Biological function: |
Circ-ACAP2 promotes CRC cell proliferation, migration, invasion, tumor growth and radioresistance, and inhibits apoptosis; circ-ACAP2 knockdown suppresses malignant phenotypes and increases radiosensitivity. |
| Molecular mechanism: |
Circ-ACAP2 acts as a molecular sponge for miR-143-3p, thereby up-regulating FZD4 and modulating Wnt/beta-catenin signalling to promote CRC progression and radioresistance. |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); radioresistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; Colony Formation Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
Circ-ACAP2/miR-143-3p/FZD4 axis provided novel potential diagnostic and therapeutic targets for CRC; silencing circ-ACAP2 might be a potential strategy to increase radiosensitivity of CRC cells. |
| Description: |
Circ-ACAP2 is up-regulated in colorectal cancer tissues and cell lines. It promotes malignant CRC phenotypes and radioresistance while suppressing apoptosis by sponging miR-143-3p to increase FZD4 expression and modulate Wnt/beta-catenin signalling. Silencing circ-ACAP2 increases radiosensitivity and suppresses tumor growth in a SW480 xenograft model. |
| Confidence score: |
0.6542 |