| Expression pattern: |
DN |
| Associated gene: |
PML, AGO2 |
| Associated microRNA: |
hsa-miR-337-3p |
| Biological function: |
Inhibits proliferation and promotes apoptosis (tumor-suppressive role) in CLL cells; its downregulation promotes cell viability. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-337-3p to regulate PML expression, thereby affecting cell viability. |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; IF (Immunofluorescence); Western Blot; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
May serve as a biomarker and therapeutic target for CLL. |
| Description: |
In CLL PBMCs, circ_0132266 is down-regulated and functions as a tumor-suppressive circRNA. It sponges miR-337-3p to relieve repression of PML, thereby inhibiting proliferation and promoting apoptosis in CLL cells. |
| Confidence score: |
0.8405 |