| Expression pattern: |
UP |
| Associated gene: |
p53, cyclin-dependent kinases 1 (CDK1), FoxO3 |
| Associated microRNA: |
- |
| Biological function: |
promotes GC progression (proliferation, migration, colony formation), inhibits stress-induced apoptosis, promotes tumor growth and lung metastasis; enhances chemosensitivity effect when combined with Dox via increased apoptosis upon circ_CEA targeting |
| Molecular mechanism: |
acts as a protein scaffold: binds p53 and CDK1, enhances p53-CDK1 association, promotes CDK1-mediated p53 phosphorylation at Ser315, reduces p53 nuclear retention and DNA binding activity, downregulates p53 pro-apoptotic target genes; also interacts with FoxO3 and suppresses its activity |
| Biological pathway or process: |
p53 signaling (inhibits); apoptosis (inhibits); proliferation (promotes); migration (promotes); metastasis (promotes); drug resistance (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RNase R Treatment; RT-qPCR; divergent primers PCR; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; EdU Staining; Wound Healing Assay; Transwell Assay; Colony Formation Assay; Annexin V/PI Flow Cytometry; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; Western Blot; In Vivo Animal Model; TUNEL; IHC (Immunohistochemistry); H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
circ_CEA may be a poor prognostic factor for GC overall survival; circ_CEA expression correlated with tumor differentiation |
| Description: |
circ_CEA (hsa_circ_0051240), derived from the CEA/CEACAM5 gene, is up-regulated in gastric cancer tissues and cell lines and acts as an oncogenic circRNA. It binds p53 and CDK1 as a scaffold to enhance CDK1-driven p53 Ser315 phosphorylation, reducing p53 nuclear retention and transcriptional activation of pro-apoptotic genes, thereby inhibiting stress/chemo-induced apoptosis and promoting tumor growth and metastasis. |
| Confidence score: |
0.8772 |