| Expression pattern: |
UP |
| Associated gene: |
HNRNPC, HOXC6 mRNA |
| Associated microRNA: |
- |
| Biological function: |
Promotes LUAD cell proliferation, migration, invasion, tumor growth, and metastasis. |
| Molecular mechanism: |
circRAPGEF5 interacts with the RNA-binding protein HNRNPC to stabilize HOXC6 mRNA, increasing HOXC6 expression and promoting HOXB2 transcription. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); mRNA stability (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Transfection; CCK8; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Bioinformatics Analysis; Actinomycin D / DRB Stability Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot |
| Clinical significance: |
These results suggest that targeting circRAPGEF5 and HOXC6 can be a promising approach for LUAD treatment. |
| Description: |
circRAPGEF5 is up-regulated in LUAD and acts as an oncogenic circRNA. Its knockdown suppresses LUAD proliferation, migration, invasion, tumor growth, and metastasis. Mechanistically, circRAPGEF5 binds HNRNPC to stabilize HOXC6 mRNA, thereby enhancing HOXC6 expression and downstream HOXB2 transcription. |
| Confidence score: |
0.7981 |