| Expression pattern: |
UP |
| Associated gene: |
CORO1C |
| Associated microRNA: |
miR-636 |
| Biological function: |
Promotes NSCLC cell proliferation, migration, invasion, tube-forming capacity, sphere formation ability and tumor formation, and inhibits apoptosis. |
| Molecular mechanism: |
circ_0025039 functions as a sponge for miR-636 and upregulates CORO1C expression through the miR-636/CORO1C axis. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); angiogenesis (promotes); stemness (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Transwell Assay; Tube Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); RNA Pull-Down; Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
circ_0025039 may serve as a therapeutic target for NSCLC. |
| Description: |
circ_0025039 is up-regulated in NSCLC tissues and cell lines and acts as an oncogenic circRNA. Its knockdown suppresses proliferation, migration, invasion, tube formation, sphere formation and xenograft tumor growth while promoting apoptosis. Mechanistically, circ_0025039 sponges miR-636 to increase CORO1C expression, supporting its potential as a therapeutic target in NSCLC. |
| Confidence score: |
0.8443 |