| Expression pattern: |
UP |
| Associated gene: |
KRT16 |
| Associated microRNA: |
- |
| Biological function: |
Promotes invasive and migratory capacity of colorectal cancer cells; enhances KRT16 protein stability; supports oxidative pentose phosphate pathway activity and metabolic reprogramming. |
| Molecular mechanism: |
circNOLC1 directly binds KRT16 through nucleotides 151 to 300, stabilizes KRT16 protein, coordinately regulates c-Met and G6PD expression, modulates c-Met nuclear translocation, and activates oxidative pentose phosphate pathway-dependent invasion and migration. |
| Biological pathway or process: |
other pathway/process (promotes); migration (promotes); invasion (promotes); metastasis (promotes); oxidative phosphorylation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RIP (RNA Immunoprecipitation); RNA Pull-Down; Bioinformatics Analysis; Transfection; Western Blot; Wound Healing Assay; Transwell Assay; IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation |
| Clinical significance: |
The circNOLC1-KRT16-c-Met-glucose-6-phosphate dehydrogenase signaling cascade is highlighted as a promising therapeutic target for colorectal cancer; circNOLC1 was previously found significantly upregulated in colon cancer liver metastasis patients. |
| Description: |
circNOLC1 acts as a pro-metastatic circRNA in colorectal cancer by directly binding KRT16 and enhancing KRT16 protein stability. The circNOLC1/KRT16 complex upregulates the c-Met/G6PD axis, maintains oxidative pentose phosphate pathway activity, and promotes colon cancer cell invasion and migration. |
| Confidence score: |
0.657 |