circRNA basic information
circBase ID: hsa_circ_0002124
Name: hsa_circ_NuSAP1
Synonym: -
Host Gene: NuSAP1 / NUSAP1
Genomic location(hg19): chr15:41667909-41669502:+
Genomic location(hg38): chr15:41375711-41377304:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; paired pericarcinomatous tissues / pericarcinomatous tissues; liver cancer tissues
Cell lines:

LO2; HepG2; Huh7; 97L; 97H; Hep3B; LM3
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: p-JNK, JNK, p-ERK, ERK, p-P38, P38, c-Myc
Associated microRNA: hsa-miR-944, hsa-miR-620, hsa-miR-577, hsa-miR-4782-3p, hsa-miR-29b-1-5p, hsa-miR-203a-5p, hsa-miR-200a-3p, hsa-miR-190b, hsa-miR-190a-5p, hsa-miR-147a, hsa-miR-141-3p, hsa-miR-135b-5p, hsa-miR-1270
Biological function: promotes HCC cell proliferation, migration and invasion; inhibits apoptosis; promotes cell cycle progression
Molecular mechanism: acts as a miRNA molecular sponge and regulates key protein expressions in the MAPK pathway
Biological pathway or process:

MAPK (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Transfection; MTT; Transwell Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis
Clinical significance:

potential biomarker for the early diagnosis and treatment of HCC
Description:

hsa_circ_0002124 is up-regulated in HCC tissues and several HCC cell lines. Functional assays in 97H cells show that it promotes proliferation, migration and invasion, facilitates cell-cycle progression, and inhibits apoptosis. Mechanistically, it is proposed to act as a miRNA sponge and enhance MAPK pathway activity (p-JNK/JNK, p-ERK/ERK, p-P38/P38, c-Myc), suggesting biomarker potential for HCC.
Confidence score:

0.7045
Other information
Title:

Circular RNA hsa_circ_0002124 promotes hepatocellular carcinoma cell proliferation through the MAPK pathway.

Journal: Translational cancer research
Published: 2019
PubMed ID: 35116769
Study type:

combined biological and clinical study
Data availability: -
Code availability: -