| Expression pattern: |
UP |
| Associated gene: |
eIF5A, SOX2, OCT-4, Nanog, CD133 |
| Associated microRNA: |
miR-486-3p, miR-587, miR-595 |
| Biological function: |
Promotes NSCLC cell proliferation, invasion/metastasis, and stemness/self-renewal; circ-EPB41 silencing induces cell-cycle arrest and suppresses tumor growth in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-486-3p, thereby relieving miR-486-3p-mediated repression of eIF5A and enhancing eIF5A-driven stemness programs. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); metastasis (promotes); stemness (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RNase R Treatment; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Cell Cycle Assay; EdU Staining; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Survival Analysis; Clinical Sample Validation |
| Clinical significance: |
High circ-EPB41 expression is associated with malignant characteristics and predicts poor overall survival in NSCLC. |
| Description: |
circ-EPB41 is up-regulated in NSCLC and mainly localized in the cytoplasm. It promotes proliferation, invasion/metastasis and cancer stemness by sponging miR-486-3p, thereby increasing eIF5A expression; high circ-EPB41 is associated with worse overall survival. |
| Confidence score: |
0.8157 |