circRNA basic information
circBase ID: hsa_circ_0005909
Name: hsa_circ_XPR1
Synonym: Circ-XPR1
Host Gene: XPR1
Genomic location(hg19): chr1:180793888-180794480:+
Genomic location(hg38): chr1:180824752-180825344:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary OS tissues; normal bone specimens; tissues adjacent to osteosarcoma tissues
Cell lines:

hFOB1.19; U2OS; U2OS/MTX300; HOS; MG63; 143B; ZOS; ZOSM; MG-63
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: DDX5
Associated microRNA: miR-214-5p
Biological function: promotes OS cell proliferation
Molecular mechanism: Circ-XPR1 acts as a miR-214-5p sponge to regulate DDX5 expression
Biological pathway or process:

proliferation (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Western Blot; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Circ-XPR1 was closely related to overall survival and disease-free survival of OS patients; high Circ-XPR1 expression was negatively correlated with overall survival and disease-free survival; Circ-XPR1/miR-214-5p/DDX5 axis may serve as a potential therapeutically relevant target for OS
Description:

Circ-XPR1/hsa_circ_0005909 is upregulated in osteosarcoma tissues and cell lines and its high expression is associated with poorer overall and disease-free survival. Functionally, Circ-XPR1 promotes osteosarcoma cell proliferation by acting as a sponge for miR-214-5p, thereby regulating DDX5 expression. The study suggests the Circ-XPR1/miR-214-5p/DDX5 axis as a potential therapeutic target in osteosarcoma.
Confidence score:

0.8256
Other information
Title:

Circ-XPR1 promotes osteosarcoma proliferation through regulating the miR-214-5p/DDX5 axis.

Journal: Human cell
Published: 2021
PubMed ID: 32920730
Study type:

combined biological and clinical study
Data availability: GSE96964; GSE65071; GSE12865; https ://doi.org/10.1007/s1357 7-020-00412 -z
Code availability: -